Terrific Broth Size:6 x 1kg Luria Broth Base or L-Broth

Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E

Compared to full Yeast Nitrogen Base medium

Difco Manual

To make YEPG-Medium add 38 ml glycerol per litre YEP medium

1 Glucose 0

Terrific Broth Size:6 x 1kg Luria Broth Base or L-BrothTerrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations